A study on cloning and expression of a fragment of DFNB59 gene encoding human pejvakin in Escherichia coli
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چکیده
منابع مشابه
CLONING AND EXPRESSION OF A HUMAN INTERFERON a2 GENE IN E. COLI
The plasmid pALCA1SIFN containing cDNA that encodes the human interferon a-2b was obtained from the ATCC(no. 531667). In this system the expression of the gene is under the control of an alcA promoter. alcA p is a specific promoter for expression of different genes in Aspergillusfilamentous. In this plasmid the coding region of IFN?-2b is preceded by the coding region of a synthetic signal ...
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Objective(s) Nonviral vector can be an attractive alternative to gene delivery in experimental study. In spite of some advantages in comparison with the viral vectors, there are still some limitations for efficiency of gene delivery in nonviral vectors. To determine the effective expression, the recombinant Escherichia coli lacZ genes were cloned into the different variants of pcDNA3.1 and the...
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ژورنال
عنوان ژورنال: TAP CHI SINH HOC
سال: 2014
ISSN: 0866-7160,0866-7160
DOI: 10.15625/0866-7160/v36n1se.4368